TaqMan SNP Genotyping Service
MGC offers both custom-designed and off-the-shelf ABI TaqMan SNP genotyping. One SNP is typed in a single experiment on 384-well-plate. Multiples of 96 samples are required, though only 92 samples can be placed by customer on each 96-well-plate; 4 open controls per 96-well-plate will be provided by MGC for internal quality control. MGC will transfer DNA samples from 96-well-plate to 384-well-plate with robot.
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ABI Pre-Designed/Validated TaqMan® SNP Genotyping Assays
Download free ABI human SNPbrowser™ and Mouse SNPbrowser™ software, to select optimal sets of TaqMan® SNP Genotyping Assays; or search at ABI website. All TaqMan® Validated and Coding SNP Genotyping Assays were converted to TaqMan® Pre-Designed SNP Genotyping Assays.
Types of Assays |
Species | Description |
TaqMan® Pre-Designed SNP Genotyping Assays |
Human |
With 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70,000 cSNPs and 160,000 validated assays. |
Mouse |
More than 10,000 mouse assay designs available for use in applications such as Speed Congenics, Genetic Mapping, and Genetic Monitoring. | |
TaqMan® Drug Metabolism Genotyping Assays |
Human |
Over 2,400 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes. |
(Source: http://www.appliedbiosystems.com)
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ABI Custom TaqMan® SNP Genotyping Assays
Both SNPs and insertion-deletions of up to six bases can be designed and ordered through ABI Custom TaqMan® SNP Genotyping Assays. Researcher can design SNPs in either human or non-human species. DNA sequence (~300 bp) and target SNP information are needed. Use ABI free File Builder software to submit sequences. ABI provides a tutorial about how to evaluate the quality of sequence information.
MGC would be happy to assist with assay design and/or ordering.